Retention mechanism of some solutes using ionic liquids as mobile phase modifier in RP-high-performance liquid chromatography (HPLC)
نویسندگان
چکیده
−Two kinds of ionic liquid, 1-Hexyl-3-methylimidazolium tetrafluoroborate ([Hmim][BF4]) and 1-Methyl-3octylimidazolium tetrafluoroborate ([Omim][BF4]), were used as additives in the linear solvation energy relationships (LSERs) model to investigate the fundamental chemical interactions governing the retention of nine aromatic compounds in acetonitrile/water mobile phases on a C18 column. The effects of the [Hmim][BF4] and [Omim][BF4] were compared and the ability of the LSERs to account for the chemical interactions underlying solute retention was shown. A comparison of predicted and experimental retention factors suggests that LSER formalism is able to reproduce adequately the experimental retention factors of the solutes studied in the different experimental conditions investigated.
منابع مشابه
Separation of Guanine and Hypoxanthine with Some Ionic Liquids in RP-HPLC
In this paper, guanine and hypoxanthine were separated with four different ionic liquids as additives for the mobile phase using reversed phase high performance liquid chromatography (RPHPLC). The ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3methylimidazolium tetrafluoroborate ([EMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) and 1-oct...
متن کاملEFFECTS OF TEMPERATURE AND PERCENTAGE OF ORGANIC MODIFIER ON RETENTION AND SELECTIVITY IN RP-HPLC USING SOLVATION PARAMETER MODEL
Effects of temperature and percentage of organic modifier were studied on retention and selectivity in RP-HPLC using solvation parameter model. The system constants were determined by multiple linear regression analysis from experimental values in the retention factor for a group of different solutes with known descriptors by computer using the program SPSS/PC. The experimental results showed t...
متن کاملRetention mechanism of selected ionic liquids on a pentafluorophenylpropyl polar phase: investigation using RP-HPLC.
This study investigated the retention mechanism of a congeneric group of imidazolium ionic liquid cations with alkyl functional groups of different lengths, an aryl substituent, and one pyridinium cation on a pentafluorophenylpropyl silica-based stationary phase. The influence of organic modifier type and content in the mobile phase on the retention mode of alkylimidazolium ionic liquid cations...
متن کاملEffect of ionic liquids as the mobile phase additives on the HPLC resolution of four active compounds from Sophora flavescens Ait.
The retention behaviour of four active compounds from Sophora Flavescens Ait using three ionic liquids as mobile phase modifiers was examined. The effect of the pH and the amount of ionic liquid modifier on the retention of these compounds was determined in methanol/water (v/v) as the mobile phase containing different ionic liquids ranging in concentration from 0.1 mmol/L to 3.0 mmol/L. The ion...
متن کاملApplication of Ionic Liquids in High Performance Reversed-Phase Chromatography
Ionic liquids, considered "green" chemicals, are widely used in many areas of analytical chemistry due to their unique properties. Recently, ionic liquids have been used as a kind of novel additive in separation and combined with silica to synthesize new stationary phase as separation media. This review will focus on the properties and mechanisms of ionic liquids and their potential application...
متن کامل